Terminator
Part:BBa_K2957068:Design
Designed by: Ethan Levy Group: iGEM19_MIT (2019-10-19)
Synthetic PolyA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4
Illegal AgeI site found at 10 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used Modular Cloning Golden Gate Type II Assembly, so we had to consider how parts came together. See more on the MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT>.
Source
This part was withdrawn from the Weiss lab database.